abstract：AIM:Osteopontin (OPN), a multifunctional protein, has been reported to be protoxicant in acetaminophen hepatotoxicity. In this study, the mechanisms underlying the detrimental role of OPN in acetaminophen toxicity were explored. METHODS:Male C57BL/6 (wild-type, WT) and OPN(-/-) mice were administered with acetaminophe...

journal_title：Acta pharmacologica Sinica

authors： He CY,Liang BB,Fan XY,Cao L,Chen R,Guo YJ,Zhao J

更新日期：2012-08-01 00:00:00

abstract：AIM:To investigate whether atorvastatin can promote formation of neurites in cultured cortical neurons and the signaling mechanisms responsible for this effect. METHODS:Cultured rat cerebral cortical neurons were incubated with atorvastatin (0.05-10 μmol/L) for various lengths of time. For pharmacological experiments,...

journal_title：Acta pharmacologica Sinica

authors： Jin Y,Sui HJ,Dong Y,Ding Q,Qu WH,Yu SX,Jin YX

更新日期：2012-07-01 00:00:00

abstract：AIM:To examine the effects of anisomycin on glioma cells and the related mechanisms in vitro. METHODS:The U251 and U87 human glioblastoma cell lines were tested. The growth of the cells was analyzed using a CCK-8 cell viability assay. Apoptosis was detected using a flow cytometry assay. The expression of proteins and ...

journal_title：Acta pharmacologica Sinica

authors： Li JY,Huang JY,Li M,Zhang H,Xing B,Chen G,Wei D,Gu PY,Hu WX

更新日期：2012-07-01 00:00:00

abstract：AIM:Pirarubicin (THP) is recently found to be effective in treating patients with advanced, relapsed or recurrent high-grade osteosarcoma. In this study, the effects of THP on the multidrug-resistant (MDR) osteosarcoma cells were assessed, and the underlying mechanisms for the disruption of cell cycle kinetics by THP w...

journal_title：Acta pharmacologica Sinica

authors： Zheng SE,Xiong S,Lin F,Qiao GL,Feng T,Shen Z,Min DL,Zhang CL,Yao Y

更新日期：2012-06-01 00:00:00

abstract：AIM:Ischemia/reperfusion is an initial triggering event that leads to gut-induced acute lung injury (ALI). In this study, we investigated whether hypoxia inducible factor-1α (HIF-1α) played a role in the pathogenesis of lung injury induced by trauma and hemorrhagic shock (T/HS). METHODS:Male Wistar rats underwent lapa...

journal_title：Acta pharmacologica Sinica

authors： Jiang H,Huang Y,Xu H,Hu R,Li QF

更新日期：2012-05-01 00:00:00

abstract：AIM:To investigate the protection of pulmonary arterial rings and cardiac myocytes of rats by raisanberine (RS), a derivative of berberine, against hypoxia injury and to elucidate the action mechanisms. METHODS:Adult SD rats were exposed to intermittent hypoxia for 17 d or 28 d. The pulmonary arterial rings were isola...

journal_title：Acta pharmacologica Sinica

authors： Gao J,Tang YQ,Dai DZ,Cheng YS,Zhang GL,Zhang C,Dai Y

更新日期：2012-05-01 00:00:00

abstract：AIM:Toxicity of retinol (vitamin A) has been previously associated with apoptosis and/or cell malignant transformation. Thus, we investigated the pathways involved in the induction of proliferation, deformation and proliferative focus formation by retinol in cultured Sertoli cells of rats. METHODS:Sertoli cells were i...

journal_title：Acta pharmacologica Sinica

authors： Gelain DP,Pasquali MA,Caregnato FF,Castro MA,Moreira JC

更新日期：2012-04-01 00:00:00

abstract：AIM:To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. METHODS:Peritoneal free cancer cells (PFCCs) were isolated from ascites specimens of gastric cancer patients. c-Met expression in PF...

journal_title：Acta pharmacologica Sinica

authors： Wang XL,Chen XM,Fang JP,Yang CQ

更新日期：2012-04-01 00:00:00

abstract：AIM:To investigate the effects of rapamycin on glutamate uptake in cultured rat astrocytes expressing N-terminal 552 residues of mutant huntingtin (Htt-552). METHODS:Primary astrocyte cultures were prepared from the cortex of postnatal rat pups. An astrocytes model of Huntington's disease was established using the ast...

journal_title：Acta pharmacologica Sinica

authors： Chen LL,Wu JC,Wang LH,Wang J,Qin ZH,Difiglia M,Lin F

更新日期：2012-03-01 00:00:00

abstract：AIM:Genetic variation in ALOX12, which encoded human 12-lipoxygenase, was found to be associated with fat mass in young Chinese men. The objective of this study was to investigate the relationship between single nucleotide polymorphisms (SNPs) and haplotypes in the ALOX15 gene and obesity-related phenotypes in Chinese ...

journal_title：Acta pharmacologica Sinica

authors： Ke YH,Xiao WJ,He JW,Zhang H,Yu JB,Hu WW,Gu JM,Gao G,Yue H,Wang C,Hu YQ,Li M,Liu YJ,Fu WZ,Zhang ZL

更新日期：2012-02-01 00:00:00

abstract：AIM:To investigate the signaling pathways involved in thrombin-induced connective tissue growth factor (CTGF) expression in rat vascular smooth muscle cells (VSMCs). METHODS:Experiments were preformed on primary rat aortic smooth muscle cells (RASMCs) and a rat VSMC line (A10). CTGF protein levels were measured using ...

journal_title：Acta pharmacologica Sinica

authors： Ko WC,Chen BC,Hsu MJ,Tsai CT,Hong CY,Lin CH

更新日期：2012-01-01 00:00:00

abstract：AIM:Epigallocatechin-3-gallate (EGCG) is the major polyphenolic constituent in green tea. The aim of this study is to investigate the effects of EGCG on proliferation and migration of the human colon cancer SW620 cells. METHODS:Proliferation and migration of SW620 cells were induced by the protease-activated receptor ...

journal_title：Acta pharmacologica Sinica

authors： Zhou F,Zhou H,Wang T,Mu Y,Wu B,Guo DL,Zhang XM,Wu Y

更新日期：2012-01-01 00:00:00

abstract：AIM:To investigate the anticancer effect of crocetin, a major ingredient in saffron, and its underlying mechanisms. METHODS:Cervical cancer cell line HeLa, non-small cell lung cancer cell line A549 and ovarian cancer cell line SKOV3 were treated with crocetin alone or in combination with vincristine. Cell proliferatio...

journal_title：Acta pharmacologica Sinica

authors： Zhong YJ,Shi F,Zheng XL,Wang Q,Yang L,Sun H,He F,Zhang L,Lin Y,Qin Y,Liao LC,Wang X

更新日期：2011-12-01 00:00:00

abstract：AIM:To evaluate the pharmacokinetics of L-threonate after single or multiple oral administrations and its safety profile in healthy Chinese volunteers. METHODS:This was an open-label, single- and multiple-dose study. The subjects were assigned to receive a single dose, 675, 2025, or 4050 mg, of calcium L-threonate (n=...

journal_title：Acta pharmacologica Sinica

authors： Wang HY,Hu P,Jiang J

更新日期：2011-12-01 00:00:00

abstract：AIM:To evaluate the pharmacokinetic interactions between theophylline and antofloxacin in vivo and in vitro. METHODS:A randomized, 5-day treatment and 3-way crossover design was documented in 12 healthy subjects. The subjects were orally administered with antofloxacin (400 mg on d 1 and 200 mg on d 2 to 5), theophylli...

journal_title：Acta pharmacologica Sinica

authors： Liu L,Pan X,Liu HY,Liu XD,Yang HW,Xie L,Cheng JL,Fan HW,Xiao DW

更新日期：2011-10-01 00:00:00

abstract：AIM:To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. METHODS:Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assist...

journal_title：Acta pharmacologica Sinica

authors： Li ZJ,Liu N,Han QD,Zhang YY

更新日期：2011-09-01 00:00:00

abstract：AIM:To determine the active ingredient of Niuchangchih (Antrodia camphorata) responsible for its anti-inflammatory effects and the relevant molecular mechanisms. METHODS:Five major antcins (A, B, C, H, and K) were isolated from fruiting bodies of Niuchangchih. Structural similarity between the antcins and 2 glucocorti...

journal_title：Acta pharmacologica Sinica

authors： Chen YC,Liu YL,Li FY,Chang CI,Wang SY,Lee KY,Li SL,Chen YP,Jinn TR,Tzen JT

更新日期：2011-07-01 00:00:00

abstract：:Human ether-a-go-go related gene (hERG) potassium (K(+)) channels play a critical role in cardiac action potential repolarization. Mutations that reduce hERG conductance or surface expression may cause congenital long QT syndrome (LQTS). However, the channels can be inhibited by structurally diverse small molecules, r...

journal_title：Acta pharmacologica Sinica

authors： Zhou PZ,Babcock J,Liu LQ,Li M,Gao ZB

更新日期：2011-06-01 00:00:00

abstract：:The hypnotic agent propofol is effective for the induction and maintenance of anesthesia. However, recent studies have shown that propofol administration is related to arrhythmias. Propofol displays both pro- and anti-arrhythmic effects in a concentration-dependent manner. Data indicate that propofol can convert supra...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Kong AL,Chen R,Qian C,Liu SW,Sun BG,Wang LX,Song LS,Hong J

更新日期：2011-06-01 00:00:00

abstract：AIM:To ascertain the effects of erlotinib on CYP3A, to investigate the amplitude and kinetics of erlotinib-mediated inhibition of seven major CYP isoforms in human liver microsomes (HLMs) for evaluating the magnitude of erlotinib in drug-drug interaction in vivo. METHODS:The activities of 7 major CYP isoforms (CYP1A2,...

journal_title：Acta pharmacologica Sinica

authors： Dong PP,Fang ZZ,Zhang YY,Ge GB,Mao YX,Zhu LL,Qu YQ,Li W,Wang LM,Liu CX,Yang L

更新日期：2011-03-01 00:00:00

abstract：:Embryonic stem (ES) cells and their differentiated progeny offer tremendous potential for regenerative medicine, even in the field of drug discovery. There is an urgent need for clinically relevant assays that make use of ES cells because of their rich biological utility. Attention has been focused on small molecules ...

journal_title：Acta pharmacologica Sinica

authors： Lou YJ,Liang XG

更新日期：2011-02-01 00:00:00

abstract：AIM:To explore the alteration of endogenous metabolites and identify potential biomarkers using metabolomic profiling with gas chromatography coupled a time-of-flight mass analyzer (GC/TOF-MS) in a rat model of estrogen-deficiency-induced obesity. METHODS:Twelve female Sprague-Dawley rats six month of age were either ...

journal_title：Acta pharmacologica Sinica

authors： Ma B,Zhang Q,Wang GJ,A JY,Wu D,Liu Y,Cao B,Liu LS,Hu YY,Wang YL,Zheng YY

更新日期：2011-02-01 00:00:00

abstract：AIM:To study the effects of 3-n-butylphthalide (NBP) on the TREK-1 channel expressed in Chinese hamster ovary (CHO) cells. METHODS:Whole-cell patch-clamp recording was used to record TREK-1 channel currents. The effects of varying doses of l-NBP on TREK-1 currents were also observed. Current-clamp recordings were perf...

journal_title：Acta pharmacologica Sinica

authors： Ji XC,Zhao WH,Cao DX,Shi QQ,Wang XL

更新日期：2011-02-01 00:00:00

abstract：AIM:To investigate the effects of the cardiotonic steroid, ouabain, on cardiac differentiation of murine embyronic stem cells (mESCs). METHODS:Cardiac differentiation of murine ESCs was enhanced by standard hanging drop method in the presence of ouabain (20 μmol/L) for 7 d. The dissociated ES derived cardiomyocytes we...

journal_title：Acta pharmacologica Sinica

authors： Lee YK,Ng KM,Lai WH,Man C,Lieu DK,Lau CP,Tse HF,Siu CW

更新日期：2011-01-01 00:00:00

abstract：AIM:To evaluate the effect of inhaled formoterol-budesonide on airway remodeling in adult patients with moderate asthma. METHODS:Thirty asthmatic patients and thirty control subjects were enrolled. Asthmatic subjects used inhaled Symbicort 4.5/160 μg twice daily for one year. The effect of formoterol-budesonide on air...

journal_title：Acta pharmacologica Sinica

authors： Wang K,Liu CT,Wu YH,Feng YL,Bai HL,Ma ES,Wen FQ

更新日期：2011-01-01 00:00:00

abstract：AIM:To investigate the anticancer mechanism of a methoxyflavanone derivative, WJ9708012, highlighting its role on a crosstalk between endoplasmic reticulum (ER) and mitochondrial stress. METHODS:Cell proliferation was examined using sulforhodamine B assay. Cell-cycle progression, Ca(2+) mobilization and mitochondrial ...

journal_title：Acta pharmacologica Sinica

authors： Kuo TC,Huang WJ,Guh JH

更新日期：2011-01-01 00:00:00

abstract：AIM:to investigate the changing resistance to nondepolarizing muscle relaxants (NDMRs) during the first month after denervation. METHODS:the denervated and innervated skeletal muscle cells were examined on days 1, 4, 7, 14, 21, and 28 after denervation. Individual denervated and innervated cells were prepared from the...

journal_title：Acta pharmacologica Sinica

authors： WANG H,YANG B,HAN GW,LI ST

更新日期：2010-12-01 00:00:00

abstract：AIM:To study the effects and mechanisms by which hyposmotic challenge modulate function of L-type calcium current (I(Ca,L)) in rat ventricular myocytes. METHODS:The whole-cell patch-clamp techniques were used to record I(Ca,L) in rat ventricular myocytes. RESULTS:Hyposmotic challenge(∼220 mosmol/L) induced biphasic c...

journal_title：Acta pharmacologica Sinica

authors： Luo AT,Luo HY,Hu XW,Gao LL,Liang HM,Tang M,Hescheler J

更新日期：2010-11-01 00:00:00

abstract：AIM:To test whether carbachol can influence endothelial barrier dysfunction induced by tumor necrosis factor (TNF)-α and whether the alpha 7 nicotinic receptor can mediate this process. METHODS:Rat cardiac microvascular endothelial cells were exposed to carbachol followed by TNF-α treatment in the presence or the abse...

journal_title：Acta pharmacologica Sinica

authors： Li YZ,Liu XH,Rong F,Hu S,Sheng ZY

更新日期：2010-10-01 00:00:00

abstract：AIM:To investigate the expression of advanced glycation end products (AGEs) and their receptor RAGE in the livers and blood vessels of rats with non-alcoholic steatohepatitis (NASH) and the effect of pentoxifylline (PTX) on liver and artery function in rats with NASH. METHODS:Sprague-Dawley rats were fed a high-fat di...

journal_title：Acta pharmacologica Sinica

authors： Wu J,Zhao MY,Zheng H,Zhang H,Jiang Y

更新日期：2010-10-01 00:00:00

abstract：AIM:To investigate the effect of epigallocatechin gallate (EGCG) on angiotensin II (Ang II)-induced stress fiber formation and hyperpermeability in endothelial cells. METHODS:Human umbilical vein endothelial cells (HUVECs) were treated with Ang II in the absence or presence of EGCG or mitogen-activated protein kinases...

journal_title：Acta pharmacologica Sinica

authors： Yang D,Liu J,Tian C,Zeng Y,Zheng YH,Fang Q,Li HH

更新日期：2010-10-01 00:00:00

abstract：:Type capital I, Ukrainian natural killer T cells (NKT cells), a subset of CD1d-restricted T cells with invariant Valphabeta TCR, are characterized by prompt production of large amounts of Th1 and/or Th2 cytokines upon primary stimulation through the TCR complex. The rapid release of cytokines implies that type capital...

journal_title：Acta pharmacologica Sinica

authors： Tan JQ,Xiao W,Wang L,He YL

更新日期：2010-09-01 00:00:00

abstract：:Tobacco use is estimated to be the largest single cause of premature death in the world. Nicotine is the major addictive substance in tobacco products. After cigarette smoking, nicotine quickly acts on its target, nicotinic acetylcholine receptors (nAChRs), which are widely distributed throughout the mammalian central...

journal_title：Acta pharmacologica Sinica

authors： Wu J

更新日期：2010-09-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is among the most lethal of human malignancies. During human multistep hepatocarcinogenesis, genomic gain represents an important mechanism in the activation of proto-oncogenes. In many circumstances, activated oncogenes hold clinical implications both as prognostic markers and targets f...

journal_title：Acta pharmacologica Sinica

authors： Chen L,Chan TH,Guan XY

更新日期：2010-09-01 00:00:00

abstract：:Brain and eye tissues are subject to a reduced version of immune surveillance, which has evolved to protect the particularly sensitive tissues from accidental bystander damage created by regular inflammatory responses. Yet, there are autoimmune diseases in both organs. This review discusses the nature of immune reacti...

journal_title：Acta pharmacologica Sinica

authors： Wekerle H,Sun DM

更新日期：2010-09-01 00:00:00

abstract：:JFCR39 is an informatic anticancer drug discovery system that utilizes a panel of 39 human cancer cells coupled with a drug-activity database. This system not only provides disease-oriented information but can also predict the mechanism of action of a given antitumor agent. Development of a phosphatidylinositol 3-kina...

journal_title：Acta pharmacologica Sinica

authors： Kong DX,Yamori T

更新日期：2010-09-01 00:00:00

abstract：AIM:To investigate whether geniposide, an iridoid glucoside extracted from gardenia jasminoides ellis fruits, inhibits cell adhesion to human umbilical vein endothelial cells (HUVECs) induced by high glucose and its underlying mechanisms. METHODS:HUVECs were isolated from human umbilical cords and cultured. The adhesi...

journal_title：Acta pharmacologica Sinica

authors： Wang GF,Wu SY,Xu W,Jin H,Zhu ZG,Li ZH,Tian YX,Zhang JJ,Rao JJ,Wu SG

更新日期：2010-08-01 00:00:00

abstract：AIM:To investigate the effects of trans-cinnamaldehyde (TCA) on the human leukemia K562 cell line and the cytotoxicity of cytokine-induced killer (CIK) cells against K562 cells. METHODS:Apoptosis, Fas expression, and mitochondrial transmembrane potential in K652 cells were analyzed using flow cytometry. K562 cells wer...

journal_title：Acta pharmacologica Sinica

authors： Zhang JH,Liu LQ,He YL,Kong WJ,Huang SA

更新日期：2010-07-01 00:00:00

abstract：AIM:To investigate the inhibitory effect of the natural product Leukamenin F on liver fibrosis and explore its potential underlying mechanisms. METHODS:Carbon tetrachloride (CCl(4))-treated mouse model in vivo and in hepatic stellate cells (HSC) in vitro were used. The effect on CCl(4)-induced liver fibrosis was studi...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Wang X,Zhang Y,Li CJ,Hu LH,Shen X

更新日期：2010-07-01 00:00:00

abstract：AIM:To investigate whether myosin light chain kinase (MLCK) contributed to the high proliferative ability of breast cancer cells. METHODS:Soft agar colony formation on the MCF-7 and LM-MCF-7 cell lines was determined. The cell cycles of MCF-7 and LM-MCF-7 were detected using flow cytometry analysis. Western blot analy...

journal_title：Acta pharmacologica Sinica

authors： Cui WJ,Liu Y,Zhou XL,Wang FZ,Zhang XD,Ye LH

更新日期：2010-06-01 00:00:00